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Description
For Normal Light Microscopy
Types A & B, with viscosities of 150 and 1250cSt respectively, meet these requirements. The greater the gap between cover glass and objective, or condenser and slide, the more desirable high viscosity becomes. For extremely large gaps, type NVH should be used. All Cargille Immersion Oils can be blended for intermediate viscosities.
37°C Immersion Oils
A number of research projects require microscopic studies at internal body temperature. Since the index/temperature relationship is inverse, increasing temperature significantly reduces the index of the immersion oil and consequently the imagery deteriorates. This problem has been resolved by the formation of Cargille 37°C Immersion Oils, which have an index of 1.515 at 37°C.
Consideration of these oils is also suggested where stage temperatures are elevated by sub-stage illuminators and high wattage projection equipment.
Special Immersion Oils
For Horizontal, Inverted and Inclined Instruments and Projection Equipment:
Higher viscosities are generally required. Type NVH meets the requirement, having a viscosity of 21,000cST.
OPTICAL & PHYSICAL PROPERTIES
| Immersion Oil Type | 37** | NVH | OVH |
| Refractive Index : | @37°C | @23°C | @23°C |
| F Line (4861 A) | 1.5238 | 1.5230 | 1.5230 |
| E Line (5461 A) | 1.5180 | 1.5178 | 1.5178 |
| D Line (5893 A) | 1.5150 | 1.5150 | 1.5150 |
| C Line (6563 A) | 1.5115 | 1.5118 | 1.5118 |
| Dispersion: | |||
| nF-nC | 0.0123 | 0.0113 | 0.0111 |
| Abbe VD | 42.0 | 45.7 | 46.3 |
| Abbe Ve | 41.7 | 45-4 | 46 |
| Temperature Coefficient (15-35°C) | -0.00035 | -0.00034 | -0.00034 |
| Stability (change in nD25°C after 24 hrs @ temp.) | |||
| at 60°C: | 0 | 0 | 0 |
| at 100°C: | 0 | 0 | 0 |
| Fluorescence (1): Short UV: | low | low | low |
| Fluorescence (1): Long UV: | low | low | low |
| Color: (Gardner) | 1 | 1 | <3 |
| Viscosity: cSt +/- 10% @ 23°C (centistokes) | 1250 high |
21,000
very high |
46,000
very high |
| Density: @ 23°C g/cc: | .917 | .919 | .918 |
| Density: (US) lb/gal | 7.73 | 7.67 | 7.66 |
| Cloud Point: | <-13°C | <-13°C | <-13°C |
| Flash Point: | 325°F | 325°F | 325°F |
| Neutralization No.: (mg KOH/g) | 0.01 max | 0.01 max | 0.01 max |
(1) Relative to Cedarwood Oil
**All values standardized at 37°C
Storage : RT
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