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Description
- Deparaffinize and hydrate to distilled water.
- Stain in Fouchet’s reagent for 10 minutes.
- Wash in running water, then in distilled water.
- Stain in Van Gieson’s stain for 2 minutes.
- Dehydrate in 95% alcohol, three changes in Coplin jars.
- Complete dehydration in absolute alcohol, three changes.
- Clear in xylene, three or four changes.
- Mount with synthetic resin.
Stain results:
| Biliverdin: | Green | |
| Collagen: | Red | |
| Muscle: | Yellow |
References:
Hall, M.J., Amer. J. Clin., 34:313 –316, 1960
Luna, L.G., (ed), Manual of Histologic Staining Methods of the AFIP, 3rd Ed. McGraw-Hill, N.Y., p. 174, c. 1968
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