Description
Opening the HPF sandwich
This video illustrates how we open te HPF sandwich to select the carrier containing the sample. Then we deposit the sandwich in the FS basket for freeze-substitution processing
https://doi.org/10.1515/mim-2024-0004
Storing high pressure frozon sample prior to freeze substitution
High pressure freezing is, in 90% of the cases, followed by Freeze Substitution. However, we never considered the FS procedure to be reliable, reproducible an …
Handling the freeze substitution basket
Exchanging Fluids during FS
How to increase freeze-substitution and electron microscopy embedding reliability
https://www.degruyter.com/document/doi/10.1515/mim-2024-0004/html
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