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Description
For Normal Light Microscopy
Types A & B, with viscosities of 150 and 1250cSt respectively, meet these requirements. The greater the gap between cover glass and objective, or condenser and slide, the more desirable high viscosity becomes. For extremely large gaps, type NVH should be used. All Cargille Immersion Oils can be blended for intermediate viscosities.
37°C Immersion Oils
A number of research projects require microscopic studies at internal body temperature. Since the index/temperature relationship is inverse, increasing temperature significantly reduces the index of the immersion oil and consequently the imagery deteriorates. This problem has been resolved by the formation of Cargille 37°C Immersion Oils, which have an index of 1.515 at 37°C.
Consideration of these oils is also suggested where stage temperatures are elevated by sub-stage illuminators and high wattage projection equipment.
Immersion Oil Type 300
Automated Hematology Systems: Use Type 300; Automated Hematology Systems depend on accurate, precisely controlled physical and optical properties of immersion oil for successful imaging and mechanical processing. Type 300 is designed and manufactured to meet the stringent requirements of this equipment, which include specialized viscosity and exacting controls for its consistency.
Immersion Oils Specifications:
OPTICAL & PHYSICAL PROPERTIES
| Immersion Oil Type | A | B | 300 |
| Refractive Index : | @23°C | @23°C | @23°C |
| F Line (4861 A) | 1.5239 | 1.5236 | 1.5238 |
| E Line (5461 A) | 1.5180 | 1.5180 | 1.5180 |
| D Line (5893 A) | 1.5150 | 1.5150 | 1.5150 |
| C Line (6563 A) | 1.5114 | 1.5115 | 1.5116 |
| Dispersion: | |||
| nF-nC | 0.0125 | 0.0119 | 0.0123 |
| Abbe VD | 41.3 | 43.2 | 41.8 |
| Abbe Ve | 41.0 | 42.8 | 41.5 |
| Temperature Coefficient (15-35°C) | -0.00033 | -0.00031 | -0.00033 |
| Stability (change in nD25°C after 24 hrs @ temp.) | |||
| at 60°C: | 0 | 0 | 0 |
| at 100°C: | 0 | 0 | 0 |
| Fluorescence (1): Short UV: | low | low | low |
| Fluorescence (1): Long UV: | low | low | low |
| Color: (Gardner) | 1 | 1 | 1 |
| Viscosity: cSt +/- 10% @ 23°C (centistokes) | 150 low |
1250 high |
300 medium |
| Density: @ 23°C g/cc: | .923 | .923 | .923 |
| Density: (US) lb/gal | 7.70 | 7.70 | 7.70 |
| Cloud Point: | <-13°C | <-13°C | <-13°C |
| Flash Point: | 325°F | 325°F | 325°F |
| Neutralization No.: (mg KOH/g) | 0.01 max | 0.01 max | 0.01 max |
(1) Relative to Cedarwood Oil
MSDS Cargille-Immersion-Oil-Type 300
Technical Data Sheet : Immersion-Oil-Type-300
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