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Description
Prepared from Sodium Phosphate Monobasic Hydrate and Sodium Phosphate Dibasic Heptahydrate. Non-Sterile. Meant for research purposes only *Meant for biological research only. Not to be used for medical or any other purposes. MSDS_19340-60 MSDS_19340-65 MSDS_19340-70 MSDS_19340-72
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PB (0.1 M phosphate buffer pH 7.2)
Reagents
Sodium phosphate dibasic
Sodium phosphate monobasic (anhydrous)
Equipment
Beakers
Graduated cylinders
Hot plate (optional; see Step 1 note)
Scale
Spatulas
Stir plate
Weigh boats
METHOD
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1. Prepare sodium phosphate dibasic stock (0.5 M) by dissolving 35.5 g of sodium phosphate dibasic in a final volume of 500 mL of H2O.
Some crystallization will occur when the solution is stored at 4ºC. Warm on a hot plate and stir until the crystals dissolve.
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2. Prepare sodium phosphate monobasic stock (0.5 M) by dissolving 30 g of anhydrous sodium phosphate monobasic in a final volume of 500 mL of H2O.
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3. Prepare 0.1 M sodium phosphate dibasic: Put 80 mL of sodium phosphate dibasic stock (0.5 M) from Step 1 in a beaker and add H2O to give a final volume of 400 mL.
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4. Prepare 0.1 M sodium phosphate monobasic: Put 30 mL of sodium phosphate monobasic stock (0.5 M) from Step 2 in a beaker and add H2O to give a final volume of 150 mL.
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5. Bring the 0.1 M sodium phosphate dibasic solution from Step 3 to pH 7.2 by adding as much as needed of the 0.1 M sodium phosphate monobasic solution from Step 4.
The resulting solution is 0.1 M phosphate buffer pH 7.2.
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