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Description
2,4,6-Trimethylpyridine N:C(CH3)CH:C(CH3)CH:CCH3 CAS #108-75-8 F.W. 121.18 Bennett & Luft., Biophys & Biochem. Cytol., 6,113 (1959). A very stable buffer, used with Osmium tetroxide; provides excellent fixation, better stability and buffering capacity. The pH can be adjusted by varying the amount of hydrochloric acid in the final volume of 200 ml. Preparation Instructions: Pour 9ml of 2N HCl; add 5.34ml of Sym-Collidine, mix well and add distilled water up to 200 ml for a pH 7.4 solution. Set consists of: – 1 amp 5.34ml Sym Collidine – 1 amp 9.0 ml of 2N HCl Storage : 0-5°C
A biologically stable buffer system for osmium tetroxide and other EM fixatives. Provides better stability and buffering capacity than traditional veronal acetate buffers.
2,4,6-Trimethylpyridine Kit
Kit Contains: 5 x 5.34ml of Collidine and 5 x 18ml of 1.0N Hcl
One kit will make 1000ml of buffer.
- Reference(s):
- Bennet, H.S. & Luft, J.H., J. Biophysics & Biochem. Cytol. 6, 113 (1959); Hendrickson, A., et al., Stain Tech., 43, 175 (1968)
- Hazards:
- Irritant , Toxic (Inhalation)
- Handling:
- Glove, chemical goggles & chemical mask or hood
- Storage:
- Store at room temperature
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