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protease inhibitor
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In biochemistry, phenylmethylsulfonyl fluoride (PMSF) is a serine protease inhibitor (serine hydrolase inactivator) commonly used in the preparation of cell… Show more (+) In biochemistry, phenylmethylsulfonyl fluoride (PMSF) is a serine protease inhibitor (serine hydrolase inactivator) commonly used in the preparation of cell lysates. PMSF does not inactivate all serine proteases. The effective concentration of PMSF is between 0.1 - 1 mM. The half-life is short in aqueous solutions (110 min at pH 7, 55 min at pH 7.5, and 35 min at pH 8, all at 25 °C). Stock solutions are usually made up in anhydrous ethanol, isopropanol, or corn oil and diluted immediately before use.C6H5CH2SO2F F.W. 174.20 - CAS #329-98-6 Show less (-) |
