Description
EMS Glycerol with DAPI and DABCO™ is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox. Fluorescence is retained during prolonged storage at 4°C in the dark. This mounting medium is fortified with DAPI which is a counter-stain for DNA. This product is to be used in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence. RNA is also stained with DAPI. Glycerol with anti-fading agent 1, 4-Diazobicyclo-(2,2,2-octane (DABCO™) is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of fluorochromes. The fluorescence is retained during prolonged storage at 4°C in the dark. Not recommended for phycoethyrin (PE), phycocyanin (PC) and allophycocyanin (APC).
Application: Immunofluorescence, confocal microscopy
Reagent: Ready to use mounting medium with anti-fading agent
Refractive Index: 1.4617
Storage: 2-8°C is recommended, Protect from light
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