PHEM Buffer 0.1M or 0.2M

Description

PHEM buffer has been used primarily for tissues and cell cultures being processed for immunocytochemical studies.  Most antigens, especially intra cellular ones, stain better using PHEM than those processed using PBS, it also has a more limited effect on biochemical reactions and enzymes. Very good preservation of cellular ultrastructure, notably microtubules, is also an advantage of PHEM use.

shelf life : 2 months

References:

1. Schliwa, Manfred, and Jonathan Van Blerkom. « Structural interaction of cytoskeletal components. » The Journal of Cell Biology 90.1 (1981): 222-235.

2. Jacqueline Montanaro: Improved ultrastructure of marine invertebrates using non-toxic buffers Academic Editor: James Reimer, published online March 31, 2016

publication : protocol

MSDS PHEM French version